From: One-step generation of error-prone PCR libraries using Gateway® technology
Name | Insert | Source | Purpose |
---|---|---|---|
pET11a-link-NGFP | None | [7] | pNGG backbone |
pNGG | Gateway cassette | This study | Library screening |
pNGG-NTAIL | NTAIL | This study | Positive control |
pNGG-Stop-NTAIL | Stop-NTAIL | This study | Negative control |
pDONR-Stop-NTAIL | Stop-NTAIL | This study | LR substrate |
pDONR-NTAIL | NTAIL | This study | epPCR substrate |
pET11a-Z-NGFP | Leucine zipper | [7] | Positive control |
pMRBAD-Z-CGFP | Leucine zipper | [7] | Positive control |
pMRBAD-link-CGFP | None | [7] | CGFP cloning backbone |
pMRBAD-XD-CGFP | XD | This study | NTAIL interacting partner |
pDEST17O/I-NTAIL | NTAIL | This study | idNTAIL PCR template |
pDEST17O/I-idNTAIL | Internally deleted NTAIL | This study | PCR2 template |