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Table 2 Plasmid constructs used in this study

From: One-step generation of error-prone PCR libraries using Gateway® technology

Name

Insert

Source

Purpose

pET11a-link-NGFP

None

[7]

pNGG backbone

pNGG

Gateway cassette

This study

Library screening

pNGG-NTAIL

NTAIL

This study

Positive control

pNGG-Stop-NTAIL

Stop-NTAIL

This study

Negative control

pDONR-Stop-NTAIL

Stop-NTAIL

This study

LR substrate

pDONR-NTAIL

NTAIL

This study

epPCR substrate

pET11a-Z-NGFP

Leucine zipper

[7]

Positive control

pMRBAD-Z-CGFP

Leucine zipper

[7]

Positive control

pMRBAD-link-CGFP

None

[7]

CGFP cloning backbone

pMRBAD-XD-CGFP

XD

This study

NTAIL interacting partner

pDEST17O/I-NTAIL

NTAIL

This study

idNTAIL PCR template

pDEST17O/I-idNTAIL

Internally deleted NTAIL

This study

PCR2 template