One-step generation of error-prone PCR libraries using Gateway® technology

Table 1 Assessment of the efficiency of E.coli transformation by different DNA species

The number of colonies obtained after E. coli transformation by a recombined acceptor¹, or by an LR reaction² is reported. T7pRos E. coli cells were either electroporated as described in "Methods", or transformed by heat-shock. Transformed cells were selected on ACplates. The results are the mean value and standard deviation of three independent transformations using the same LR reaction
¹25 fmoles of pNGG-N_TAIL (Table 2). ²using 25 fmoles of non recombined acceptor plasmid (pNGG, Table 1) and 100 fmoles of linear N_TAIL coding sequence flanked by att L recombination sites (Figure 1, stage 1, right panel)

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