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Figure 2 | Microbial Cell Factories

Figure 2

From: In vivo modification of tyrosine residues in recombinant mussel adhesive protein by tyrosinase co-expression in Escherichia coli

Figure 2

Over-expression and purification of co fp-151. (A) SDS-PAGE analysis for the expression of fp-151 without and with tyrosinase. The fp-151 protein was expressed alone using the pET-fp151 plasmid (fp-151 expression w/o tyrosinase) or co-expressed using the two plasmids pACYC-Tyr-438 and pET-fp151 (fp-151 expression w/ tyrosinase) in E. coli. Lanes: M, protein molecular weight marker; S, soluble supernatant fraction; IS, insoluble cell debris fraction. (B) SDS-PAGE analysis of co fp-151 purified by Ni-NTA affinity chromatography. Lanes: M, protein molecular weight marker; P, purified sample. (C) Lyophilized powder of the purified co fp-151.

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Microbial Cell Factories

ISSN: 1475-2859

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