Table 4 Mutant genotypes and corresponding site-specific primer sequences (F, forward; R, reverse) used for verification ^a

Δpgi

Δpgi- F

5′- GATCACGCGTATCCCTTCTCCGGCATC- 3′

Δpgi- R

5′- GATCTCTAGATCCAGCGACACGAATAATC- 3′

ΔpurA

ΔpurA- F

5′- GATCTCTAGAATGGATCGGATGTGACG- 3′

ΔpurA- R

5′- GATCACGCGTCAATCGGTCAACCTGGT- 3′

ΔguaB2

ΔguaB2- F

5′- GATCACGCGTGAGTTTCTACCGGAGGA- 3′

ΔguaB2- R

5′- GATCTCTAGATCAGACTGGAAGTAACG- 3′

purF ^K348Q

purF^K348Q- F

5′- GATCACGCGTGATTGCGGACTGGTTAC- 3′

purF^K348Q- R

5′- GATCTCTAGACTCCTGCTGCTGCGTATG- 3′

K348Q- 1

5′- GGCCAAGGCATGGTCCAG AACGCCTACGTTGGC- 3′

K348Q- 2

5′- GCCAACGTAGGCGTTCTG GACCATGCCTTGGCC- 3′