Table 3 Genetically modified organisms constructed in the present study: Mutant strains of C. glutamicum based on the wild type strain (ATCC 13032) were constructed by site-directed mutagenesis
Strain | Genotype | Reference |
|---|---|---|
C. glutamicum ATCC 13032 | wild type | American Type Culture Collection |
C. glutamicum Δpgi | deletion of the pgi gene (NCgl0817) | This work |
C. glutamicum ΔpurA | deletion of the purA gene (NCgl2669) | This work |
C. glutamicum ΔguaB2 | deletion of the guaB2 gene (NCgl0578) | This work |
C. glutamicum purF K348Q | Nucleotide exchange K348Q in the purF gene (NCgl2495) | This work |
C. glutamicum ΔpurA ΔguaB2 | double deletion of purA and guaB2 genes | This work |
C. glutamicum ΔpurA ΔguaB2 purF 348 Δpgi | deletion of purA, guaB2 and pgi genes and allelic amino acid substitution K348Q in the purF gene | This work |
E. coli DH5α | F−endA1, hsdR17(rk−mk+) supE44, thi-1λ− recAI gyrA96 relA1 Φ80ΔlacAm15 | [48] |
E. coli NM522pTC | supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (r K−m K+) [F′ proAB lacI qZΔM15] | Stratagene |