Figure 4
XADH formation for C. glutamicum ATCC 13032, C. glutamicum Δpgi, C. glutamicum ΔpurA ΔguaB2 and C. glutamicum ΔpurA ΔguaB2 purFK348Q Δpgi . Glyceraldehyde 3-phosphate dehydrogenase (GAP-DH), pyruvate dehydrogenase (Pyr-DH), α-ketoglutarate dehydrogenase (AKG-DH) and malate dehydrogenase (MAL-DH) were considered as NADH supplying reactions and succinate dehydrogenase (SUCC-DH) as FADH2 supplying reaction. Anabolically derived NADH was considered with 3.2 mmol gCDW-1[40]