Table 1 Bacterial strains and plasmids used in this study
Strain or plasmid | Characteristic(s) | Source or reference |
|---|---|---|
Lactobacillus plantarum | ||
NZ7100 | WCFS1 lp_0076::nisRK | [46] |
TR0010 | NZ7100 lp_2645 (acm2)::lox72 | This work |
TR0011 | NZ7100 lp_3093 (lys2)::lox72 | This work |
TR0012 | NZ7100 lp_1138 (acm1)::lox72 | This work |
TR0013 | NZ7100 lp_1158 (lys1)::lox72 | This work |
TR0014 | NZ7100 lp_1982 (lytH)::lox72 | This work |
TR006 | NZ7100 lp_3421 (lytA)::lox66-P32-cat-lox71 | This work |
TR0015 | NZ7100 lp_2162 (lytB)::lox72 | This work |
TR0016 | NZ7100 lp_1242 (lytD)::lox72 | This work |
TR0017 | NZ7100 lp_0302 (mltA)::lox72 | This work |
TR0018 | TR0011 lp_2645 (acm2)::lox72 | This work |
Escherichia coli | ||
DH5α | Cloning host; F- ϕ80 lacZ∆ M15 ∆(lacZYA-argF) U169 endA1 recA1 hsdR17 (r k -m k +) phoA supE44 thi-1 gyrA96 relA1 λ | [47] |
Lactococcus lactis | ||
NZ3900 | MG1363 derivative, pepN::nisRK | [30] |
Plasmids | ||
pNZ5319 | Cmr Emr; pACYC184 derivative containing the cat gene under the control of the P32 constitutive promoter of Lactococcus lactis (lox66-P32-cat-lox71 cassette) | [29] |
pNZ5348 | Emr; Cre expression vector | [29] |
pNZ8048 | Cmr; shuttle vector containing P nisA promoter and start codon in NcoI site | [48] |
pGITR001 | Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_2645 (acm2) | This work |
pGITR002 | Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_3093 (lys2) | This work |
pGITR003 | Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_1138 (acm1) | This work |
pGITR004 | Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_1158 (lys1) | This work |
pGITR005 | Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_1982 (lytH) | This work |
pGITR006 | Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_3421 (lytA) | This work |
pGITR007 | Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_2162 (lytB) | This work |
pGITR008 | Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_1242 (lytD) | This work |
pGITR009 | Cmr Emr; pNZ5319 containing both upstream and downstream homology fragments from lp_0302 (mltA) | This work |
pGITR0010 | Cmr; pNZ8048 derivative containing acm2 (lp_2645) gene in transcriptional fusion | This work |
pGITR0011 | Cmr; pNZ8048 derivative containing lys2 (lp_3093) gene in transcriptional fusion | This work |