Figure 5

Autolysis of L. plantarum and its mutant derivatives in presence of Triton X-100 (0.05%). Wild type (WT) is represented by a line, acm2 mutant (Acm2^–) by open triangles, lys2 mutant (Lys2^–) by crosses, lytA mutant (LytA^–) by black triangles, lytH mutant (LytH^-) by open circles, and double acm2 lys2 mutant (Acm2^– + Lys2^–) by black circles. Cells were grown in MRS (A), CDM (B) and SCDM (C). Mean values ± standard deviations (n=3). OD₆₀₀ values of the acm2 mutant (MRS, CDM, and SCDM), lys2 mutant (SCDM), lytA mutant (MRS), lytH mutant (CDM and SCDM) and double acm2 lys2 mutant (MRS, CDM, and SCDM) are all significantly different from the WT between 4 and 15 h. OD₆₀₀ values of the acm2 lys2 mutant (SCDM) are all significantly different from the acm2 mutant (SCDM) between 12 and 15 h. Significance is based on Student’s t test with a P value of <0.001.