Skip to main content

Table 1 Expression profiles of E . coli DH5α cells containing the indicated constructs

From: Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette

Combinations xylS Pm 5'-UTR Induced* Uninduced* Reference
wild-type wild-type wild-type wild-type 0.2 - 0.4 0.005 - 0.01 This study
comP wild-type ML2-5 wild-type 1 – 2 0.005 - 0.01 [15]
comU wild-type wild-type H39 2 – 3 0.06 - 0.08 This study
comX StEP-13 wild-type wild-type 1 – 2 0.02 - 0.03 [17]
comPU wild-type ML2-5 H39 5 – 6 1 - 1.5 This study
comXP StEP-13 ML2-5 wild-type 3 – 4 0.3 - 0.4 This study
comXU StEP-13 wild-type H39 12 – 13 0.8 - 0.9 This study
comXPU StEP-13 ML2-5 H39 15 – 20 3 - 4 This study
  1. * Phenotypes are given as ampicillin resistance levels (mg mL-1) under induced (0.1 mM m-toluate) and uninduced conditions.