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Table 1 Expression profiles of E . coli DH5α cells containing the indicated constructs

From: Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette

Combinations

xylS

Pm

5'-UTR

Induced*

Uninduced*

Reference

wild-type

wild-type

wild-type

wild-type

0.2 - 0.4

0.005 - 0.01

This study

comP

wild-type

ML2-5

wild-type

1 – 2

0.005 - 0.01

[15]

comU

wild-type

wild-type

H39

2 – 3

0.06 - 0.08

This study

comX

StEP-13

wild-type

wild-type

1 – 2

0.02 - 0.03

[17]

comPU

wild-type

ML2-5

H39

5 – 6

1 - 1.5

This study

comXP

StEP-13

ML2-5

wild-type

3 – 4

0.3 - 0.4

This study

comXU

StEP-13

wild-type

H39

12 – 13

0.8 - 0.9

This study

comXPU

StEP-13

ML2-5

H39

15 – 20

3 - 4

This study

  1. * Phenotypes are given as ampicillin resistance levels (mg mL-1) under induced (0.1 mM m-toluate) and uninduced conditions.