Figure 5

Western Blot (a) and gm - csf transcript amounts (b) for extra-chromosomally expressed combination constructs. (a) 1 and 4: wt plasmid with elevated copy number (3-4x); 2 and 5: comPU; 3 and 6 : comXPU; total cell protein was loaded on the gel (b) values are relative to the wild-type plasmid with elevated copy number (arbitrarily set to 1). The values are the average of at least two biological replicas. E. coli RV308 was used as host.