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Figure 2 | Microbial Cell Factories

Figure 2

From: Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette

Figure 2

Map of plasmid pTA16. Pm, positively regulated promoter; xylS, gene encoding Pm activator; Apr(bla), ampicillin resistance gene encoding β- lactamase; Kmr, kanamycin resistance gene; trfA, gene encoding the essential replication protein; oriV, origin of vegetative replication; oriT, origin of conjugal transfer; t1, rrnBTlT2 bidirectional transcriptional terminator; t2, bidirectional transcriptional terminator. Restriction sites shown are unique: 1-Sfil, 2-Agel, 3-Sacl, 4-Xbal, 5-BspLUlll, 6-Ndel, 7-Notl, 8-BamH1, 9-Kpn1.

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