Figure 1

Sequence profiles of the variants that are used in this study. Restriction sites shown are unique. Nucleotides in lower case indicate randomly mutagenized bases, and introduced mutations are indicated for each construct (amino acid substitutions for XylS, and base changes for Pm and 5'-UTR variants). Identical nucleotides are indicated by dots, deletion mutations are indicated by short horizontal lines. Transcriptional and translational start sites are written in bold face and indicated with filled circles. SD is the putative Shine-Dalgarno sequence.