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Table 1 Some of the highest pDNA concentrations reached in batch cultivations

From: Engineering Escherichia coli to increase plasmid DNA production in high cell-density cultivations in batch mode

Reference

Strain used

Cultivation medium

Carbon source (concentration)

pDNA concentration reached (mg/L)

[36]

DH5α

Defined, supplemented with glutamate

Glycerol (52 g/L)

45

[37]

JM109

Defined, supplemented with 20 aminoacids and nucleotides

Glucose (5 g/L)

60

[38]

HB101

Complex, supplemented with yeast extract, casaminoacids, torula yeast RNA and RNase A

Glucose (20 g/L)

109

[39]

DH5α

Complex, supplemented with casein peptone and yeast extract

Sucrose (10 g/L) and glycerol (10 g/L)

52

[20]

VH33

Mineral

Glucose (100 g/L)

40

This work

VH33 Δ (recA deoR nupG)

Mineral

Glucose (100 g/L)

186