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Table 3 Plasmids used in this study

From: An internal deletion in MTH1 enables growth on glucose of pyruvate-decarboxylase negative, non-fermentative Saccharomyces cerevisiae

Plasmid

Characteristic

Reference

pENTR/D-TOPO

Gateway entry clone

Invitrogen, USA

pvv214

2μ ori, URA3, P PGK1 -ccdB-T CYC1

[59]

pENTR-D-TOPO-MTH1

Gateway entry clone, MTH1

This work

pEXp214-MTH1.2

2μ ori, URA3, P PGK1 - MTH1-ΔT -TCYC1

This work

pRS406

Integration plasmid, URA3

[56]

pUD143

Integration plasmid, URA3, P MTH1 - MTH1-ΔT -TMTH1

This work