Figure 2

Growth of S. cerevisiae strains with different MTH1 alleles on synthetic medium agarose plates with 20 g l^-1glucose as the sole carbon source (left plate), 20 g l^-1glucose supplemented with 0.3% (v/v) ethanol (middle plate) or 2% (v/v) glycerol and 0.3% (v/v) ethanol (right plate). The strains used were: IMI078 (Pdc^-MTH1-ΔT), IMI082 (Pdc^-), IMI083 (evolved Pdc^-), IMZ104 (Pdc^- overexpression MTH1), IMZ103 (Pdc^- empty plasmid), CEN.PK113-7D (Pdc⁺ reference). Plates were incubated at least 3 days at 30° C.