Table 4 Oligonucleotide sequences
Primers for construction and verification of plasmids | |
|---|---|
pET-F | TAAAGGGAACAAAAGCTGGAGCT |
pET-R | GCCAAGCTT ACGGATGTGAACGTAGGTAC?? |
pET-HF | GCCAAGCTT ACGGATGTGAACGTAGGTAC |
qPCR-OmpA-S | GTATGGCGTGCAGACACTAA |
qPCR-OmpA-A | ACTGGTATTCCAGACGGGTAG |
qPCR-phaC-S | TGATCCAGTACCGTCCGATGT |
qPCR-phaC-A | GAAGACAGACCCCATTCACG |
02SF | 5’- ATTGAGCTC GTATTTTGGATGATAACGAGGAGGTAAATAATGCGAGACAAGCCCAATAG a |
02SR | 5’- AATTACTAGT CCTCAGCGGATATGCACGTAGG -3’ |
YXXHF | 5’- ATAATTGAGCTC CACGTCGACTTATCTCGAGACTG -3’ |
YXXF | 5’- ATAATTGAGCTC GTATTTTGGATGATAACGAGGAG -3’ |
YXXR | 5’- ATAATTACTAGT CCTTAACGGATGTGAACGTAGGT -3’ |
Tes02F | 5’- ATTCTGTGGATAACCGTATTACC -3’ |
Tes02R | 5’- GCACACCTTGTTGATGGTCATGG -3’ |