Production of copolyesters of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by E. coli containing an optimized PHA synthase gene

Table 3 Scl and mcl PHA accumulation by recombinant E. coli LS5218 harboring pYC2OH grown in mixtures of gluconate and dodecanoate ^a

Concentration ofgluconate (g/l)	CDW (g/l)	PHA content (wt%)	PHA composition (mol %)
Concentration ofgluconate (g/l)	CDW (g/l)	PHA content (wt%)	3HB	3HHx	3HO	3HD	3HDD
5	3.73±0.02	4.18±0.16	52.39±3.40	5.16±0.54	20.24±1.11	14.89±1.75	7.32±0.70
10	3.47±0.33	3.99±0.35	70.11±3.12	4.50±0.31	14.16±0.72	7.01±0.62	4.22±0.08
20	2.10±0.10	3.36±0.18	96.33±0.78	ND	3.67±0.78	ND	ND

^a Cells were cultivated at 37°C and 200 rpm for 48 h as described in “Methods”. Mixed carbon sources consisting of different concentrations of gluconate and 8 g/l dodecanoate were added to the MS medium. Three parallel experiments were conducted for each datum.

ISSN: 1475-2859