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Table 2 Scl and mcl PHA production by E. coli LS5218 harboring different PhaC2 Ps s a

From: Production of copolyesters of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by E. coli containing an optimized PHA synthase gene

Plasmid CDWb(g/l) PHA contentc(wt%) Significantdifferenced PHA composition (mol %)e
3HB 3HHx 3HO 3HD 3HDD
pYC2 3.23±0.03 0.81±0.06 - 32.35±1.16 ND f 10.61±2.84 57.04±3.66 ND
pYC2QK 3.15±0.12 1.41±0.06 0.019 46.00±2.84 ND 6.90±0.53 47.10±2.82 ND
pYC2QKST 3.13±0.06 1.79±0.10 0.008 41.59±3.43 ND 6.28±0.56 49.93±2.77 2.21±0.09
pYC2O 3.39±0.04 3.40±0.05 0.004 39.49±0.36 5.93±0.05 6.97±0.06 45.33±0.24 2.27±0.02
pYC2OH 3.59±0.11 3.93±0.11 0.004 39.59±0.23 5.80±0.12 6.91±0.12 45.46±0.27 2.24±0.07
  1. a Cells were cultivated at 37°C and 200 rpm for 48 h as described in “Methods”. 8 g/l dodecanoate were added to the MS medium. Three parallel experiments were conducted for each datum.
  2. b CDW: cell dry weight.
  3. c PHA content: PHA contents are given as mass percentage of CDW.
  4. d Significant difference: p value calculated by student’s t-test, indicating the significant difference between the respective and the previous PHA contents.
  5. e 3HB, 3-hydroxybutyrate; 3HHx, 3-hydroxyhexanoate; 3HO, 3-hydroxyoctanoate; 3HD, 3-hydroxydecanoate; 3HDD, 3-hydroxydodecanoate.
  6. f ND: not detected.