Production of copolyesters of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by E. coli containing an optimized PHA synthase gene

Table 2 Scl and mcl PHA production by E. coli LS5218 harboring different PhaC2 _Ps s ^a

Plasmid	CDW^b(g/l)	PHA content^c(wt%)	Significantdifference^d	PHA composition (mol %)^e
Plasmid	CDW^b(g/l)	PHA content^c(wt%)	Significantdifference^d	3HB	3HHx	3HO	3HD	3HDD
pYC2	3.23±0.03	0.81±0.06	-	32.35±1.16	ND ^f	10.61±2.84	57.04±3.66	ND
pYC2QK	3.15±0.12	1.41±0.06	0.019	46.00±2.84	ND	6.90±0.53	47.10±2.82	ND
pYC2QKST	3.13±0.06	1.79±0.10	0.008	41.59±3.43	ND	6.28±0.56	49.93±2.77	2.21±0.09
pYC2O	3.39±0.04	3.40±0.05	0.004	39.49±0.36	5.93±0.05	6.97±0.06	45.33±0.24	2.27±0.02
pYC2OH	3.59±0.11	3.93±0.11	0.004	39.59±0.23	5.80±0.12	6.91±0.12	45.46±0.27	2.24±0.07

^a Cells were cultivated at 37°C and 200 rpm for 48 h as described in “Methods”. 8 g/l dodecanoate were added to the MS medium. Three parallel experiments were conducted for each datum.
^b CDW: cell dry weight.
^c PHA content: PHA contents are given as mass percentage of CDW.
^d Significant difference: p value calculated by student’s t-test, indicating the significant difference between the respective and the previous PHA contents.
^e 3HB, 3-hydroxybutyrate; 3HHx, 3-hydroxyhexanoate; 3HO, 3-hydroxyoctanoate; 3HD, 3-hydroxydecanoate; 3HDD, 3-hydroxydodecanoate.
^f ND: not detected.

ISSN: 1475-2859