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Table 1 Bacterial strains and plasmids

From: Production of copolyesters of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by E. coli containing an optimized PHA synthase gene

Strains or plasmids Relevant characteristics Source or reference
Strains   
E. coli JM109 recA1, endA1, gyrA96, thi, hsdR17, supE44, relA1, Δ(lac proAB)/F’ [traD36, proAB+, lacqlacZΔ M15] TaKaRa (Dalian, China)
E. coli LS5218 atoC fadR [37]
Plasmids   
pBHR69 pBluscript SK- derivative, phbA Re and phbB Re cloned from R. eutropha [38]
pCJY02 pBluscript SK- derivative, phbA Re and phbB Re cloned from R. eutropha phaC2 Ps cloned from P. stutzeri 1317 [39]
pSXWQK pBBR1MCS-2 derivative, mutated phaC2 Ps Gln482→Lys (CAG→AAG) [20]
pSXWQKST pBBR1MCS-2 derivative, double mutated phaC2 Ps Ser326→Thr (AGC→ACC) Gln482→Lys (CAG→AAG) [20]
pETC2 Wild type phaC2 Ps inserted into pET28a This study
pETC2QK Mutated phaC2 Ps inserted into pET28a This study
pETC2QKST Double mutated phaC2 Ps inserted into pET28a This study
pETC2O Codon optimized phaC2 Ps QKST inserted into pET28a This study
pETC2OH Codon optimized phaC2 Ps QKST with hairpin sequence inserted into pET28a This study
pYC2 pBHR69 derivative, wild type phaC2 Ps of P. stutzeri 1317, plus phbA Re and phbB Re This study
pYC2QK Mutated phaC2 Ps inserted into pBHR69 This study
pYC2QKST Double mutated phaC2 Ps inserted into pBHR69 This study
pYC2O Codon optimized phaC2 Ps QKST inserted into pBHR69 This study
pYC2OH Codon optimized phaC2 Ps QKST with hairpin sequence inserted into pBHR69 This study