Table 1 Bacterial strains and plasmids
Strains or plasmids | Relevant characteristics | Source or reference |
|---|---|---|
Strains | Â | Â |
E. coli JM109 | recA1, endA1, gyrA96, thi, hsdR17, supE44, relA1, Δ(lac proAB)/F’ [traD36, proAB+, lacqlacZΔ M15] | TaKaRa (Dalian, China) |
E. coli LS5218 | atoC fadR | [37] |
Plasmids | Â | Â |
pBHR69 | pBluscript SK- derivative, phbA Re and phbB Re cloned from R. eutropha | [38] |
pCJY02 | pBluscript SK- derivative, phbA Re and phbB Re cloned from R. eutropha phaC2 Ps cloned from P. stutzeri 1317 | [39] |
pSXWQK | pBBR1MCS-2 derivative, mutated phaC2 Ps Gln482→Lys (CAG→AAG) | [20] |
pSXWQKST | pBBR1MCS-2 derivative, double mutated phaC2 Ps Ser326→Thr (AGC→ACC) Gln482→Lys (CAG→AAG) | [20] |
pETC2 | Wild type phaC2 Ps inserted into pET28a | This study |
pETC2QK | Mutated phaC2 Ps inserted into pET28a | This study |
pETC2QKST | Double mutated phaC2 Ps inserted into pET28a | This study |
pETC2O | Codon optimized phaC2 Ps QKST inserted into pET28a | This study |
pETC2OH | Codon optimized phaC2 Ps QKST with hairpin sequence inserted into pET28a | This study |
pYC2 | pBHR69 derivative, wild type phaC2 Ps of P. stutzeri 1317, plus phbA Re and phbB Re | This study |
pYC2QK | Mutated phaC2 Ps inserted into pBHR69 | This study |
pYC2QKST | Double mutated phaC2 Ps inserted into pBHR69 | This study |
pYC2O | Codon optimized phaC2 Ps QKST inserted into pBHR69 | This study |
pYC2OH | Codon optimized phaC2 Ps QKST with hairpin sequence inserted into pBHR69 | This study |