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Figure 3 | Microbial Cell Factories

Figure 3

From: Production of copolyesters of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by E. coli containing an optimized PHA synthase gene

Figure 3

PHB accumulation of E. coli JM109 expressing different PhaC2 Ps s using gluconate as carbon source. Data represent results for three independent experiments ± standard deviations. The cultivation was conducted in 500 ml shake flasks each having 50 ml MS medium containing 20 g/l gluconate and 100 mg/l thiamine. IPTG (0.1 mol/l) was used as an inducing agent for lac promoter and was added at 6 h after inoculation. After 48 h cultivation, strains harboring pYC2OH showed the highest CDW and PHB content (30 wt%, approximately 16-fold of the strains harboring pYC2), and the effect of codon optimization and hairpin stabilization was about 2.6-fold compared to the strain harboring pYC2QKST (p < 0.01).

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