Table 1 Strains and Plasmids used in this study

pMAF10

HA-tagged PglB_Cj cloned in pMLBAD, Tmp^R

(11)

pMH5

Soluble periplasmic hexa-His-tagged AcrA under control of Tet promoter, in pACYC, Cm^R

(11)

YeO9-c-OC (OC-)

Δ(wzx-wbcL)::KmGB; OC negative; derivative of Ruokola/71-c

(35)

YeO9-OC-R (OC-/HP-)

Phage ∅R1-37-resistant spontaneous OC-negative derivative of YeO9-R1

(35)

YeO9-R1 (HP-)

Δper::KmGB; rough (O-antigen negative); Km^r derivative of Ruokola/71

(32,35)

Ruokola/71-c (WT)

Spontaneous virulence plasmid-cured derivative of Ruokola/71

(32)