Figure 1
Bacterial colonies grown on YMA containing Congo red. A) Different color intensities of Congo red binding in different strain, from left to right: R. cellulosilyticum ALA10B2 T (+++), R. leguminosarum bv. trifolii ANU843 (++), R. hainanense I66T (+), and E. kostiense LMG 19227 T (w). B-C) Congo red uptake by colonies of root-nodule legume symbionts: (B) representative type strains of different genera: 1. R. phaseoli ATCC 14482T, 2. E. meliloti ATCC 9930T, 3. M. loti ATCC 33669T, 4. B. elkanii LMG 6134T, 5. P. trifolii PETPO2 T, 6. A. caulinodans ORS 571T, 7. D. neptuniae J1T and (C) representative type strains of genus Rhizobium: 1. R. hainanense I66T, 2. R. leguminosarum ATCC10004T, 3. R. galegae ATCC 43677T, 4. R. etli CFN 42T, 5. R. lusitanum P1-7T, 6. R. loessense CCBAU 7190BT, 7. R. giardinii H152T, 8. R. mongolense USDA 1844T, 9. R. indigoferae CCBAU 71042T, 10. R. tropici CIAT 899T, 11. R. yanglingense CCBAU 71623, 12. R. huautlense SO2T, 13. R. gallicum R602spT, 14. R. cellulosilyticum ALA10B2T and (D) wild-type strain of Rhizobium leguminosarum bv. trifolii ANU843 (wt) and its derivatives ANU843ΔC2 (ΔC2), ANU843C2+ (C2+), ANU843ΔC2 complemented (ΔC2comp) and ANU843EV (EV).