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Figure 1 | Microbial Cell Factories

Figure 1

From: Capsid protein expression and adeno-associated virus like particles assembly in Saccharomyces cerevisiae

Figure 1

Schematic representation of expression cassettes constructed in this study. a) In the plasmid YEplacp40Cap, entire AAV2 cap gene is under control of AAV2 p40 promoter. b) the YEplacRepCap plasmid contains rep and cap gene in wt configuration. c) In the vector pYESCap, the entire cap gene is under control of Gal1 promoter. d) In the plasmid pYESIntronCap, the cap gene is placed under control of Gal1 promoter and the AAV2 intron sequence upstream the promoter. e) In the vector pYESVP1KM, the VP1 coding sequence, placed under control of Gal1 promoter, was mutated in three sites. The point mutations were made in position 11 to eliminate an out of frame ATG codon (indicated as M1 in the scheme), in position 21 and 24 to inactivate the major AAV splice acceptor site (indicated as M2 in the schemes). A yeast Kozak like sequence was cloned upstream the VP1 start codon.

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