Figure 4
From: Extensive exometabolome analysis reveals extended overflow metabolism in various microorganisms
Impact of sampling procedure on the cell integrity of E. coli WT. (A) Different sample treatments for cell washing and separation. (B) Untargeted GC-TOF-MS analysis of resulting cell-free supernatants. Here the mass peaks detected in the washing, filtrate and centrifugate samples are due to derivatized compounds of the washing solution. (C) Targeted LC-ESI-MS/MS analysis of resulting cell-free supernatants and cell extracts focusing on specific sugar phosphates from glycolysis. Abbreviations: G6P, glucose-6-phosphate; F6P, fructose-6-phosphate; FBP, fructose-1,6-bisphosphate; PEP, phosphoenolpyruvate.