Figure 3

Effects of Mn²⁺on the activity and thermal stability of the Pyrococcus iPGM and Thermus iPGM. The crude extract of recombinant E. coli with Pyrococcus iPGM (orange circle) or Thermus iPGM (green circle) was incubated at 50°C in 50 mM HEPES-NaOH (pH 7.0) with (solid circle) or without (empty circle) 0.5 mM MnCl₂. The residual iPGM activities were determined under standard assay conditions except that MnCl₂ was absent in the assay mixture for the enzyme solutions incubated without MnCl₂.