Figure 2
Schematic illustration of design of chimeric Embden-Meyerhof (EM) pathway. The bacterial/eukaryotic classic EM pathway (left), modified EM pathway of Thermococcales archaea (center), and chimeric EM pathway constructed in this study (right) are illustrated. Reactions catalyzed by enzymes uniquely involved in the archaeal modified EM pathway are indicated by green arrows. Abbreviations used: G6P, glucose-6-phosphate; F6P, fructose-6-phosphate; FBP, fructose-1,6-bisphosphate; GAP, glyceraldehyde-3-phosphate; DHAP, dihydroxyacetone phosphate; 1,3-BPG, 1,3-bisphosphoglycerate; 3-PG, 3-phosphoglycerate; 2-PG, 2-phosphoglycerate; PEP, phosphoenolpyruvate; GK, glucose kinase; PGI, glucose-6-phosphate isomerase; PFK, 6-phosphofructokinase; FBA, fructose-bisphosphate aldolase; TIM, triosephosphate isomerase; PGM, phosphoglycerate mutase; ENO, enolase; PK, pyruvate kinase; MLDH, malate/lactate dehydrogenase, GAPOR, glyceraldehyde-3-phosphate ferredoxin oxidoreductase; GAPN, non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PGK, phosphoglycerate kinase; Fdox, oxidized ferredoxin; and Fdred, reduced ferredoxin.