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Table 1 Catalytic performance of Acetobacter sp. CCTCC M209061 cells immobilized in various matrices

From: Immobilization of Acetobacter sp. CCTCC M209061 for efficient asymmetric reduction of ketones and biocatalyst recycling

Immobilizing matrix

Time (min)a

Catalyst load (mg-dw/g)

v0(μ mol/min)

Specific activity (μ mol/min/g dw cells)

Specific activity (μ mol/min/g ww catalyst)

Yield (%)

e.e.(%)

None (free cells)

90

N/Ab

1.62

40.54

N/A

97.0

>99%

Agar

120

5.79

1.08

27.09

0.16

95.1

>99%

κ-Carrageenan

120

5.56

1.18

29.61

0.16

97.3

>99%

Ca-alginate

90

7.81

1.42

34.78

0.27

97.5

>99%

Ba-alginate

90

7.35

1.41

34.79

0.26

97.3

>99%

Zn-alginate

120

7.69

1.04

25.96

0.20

98.5

>99%

Cu-alginate

120

7.46

0.67

16.68

0.12

73.4

>99%

  1. Conditions: 8 mL TEA-HCl buffer (100 mmol/L, pH 5.0) containing 130 mmol/L of isopropanol and 5.0 mmol/L of 4′-chloroacetophenone, 0.24 g wet cells or immobilized matrix with 0.24 g wet cells, T = 30°C, 160 rpm.
  2. aReaction time to achieve the maximum yield.
  3. bN/A, not applicable.