Figure 6

The repeated asymmetric reduction of 4′-chloroacetophenone with the complex-immobilized cells and free cells . Repeated actions were carried out with the free cells (○) and the complex immobilized cells (▪) in 8 mL TEA-HCl buffer (100 mmol/L, pH 5.0) containing 130 mmol/L of isopropanol and 5.0 mmol/L of 4′-chloroacetophenone, 4.7 g ww immobilized biocatalyst or 0.48 g wet free cells, 30°C, 160 rpm, reaction time for each batch was 60 min. Arrow: recultivation of 24 h was carried out as described in Materials and methods.