Figure 5
From: A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli
Western blot showing distribution of SefA and H:gm from pAIDA1 in cellular fractions. Left: Detection through an anti-His6 antibody conjugated with horseradish peroxidase. Lanes 1 and 6: Size marker. Lanes 2 and 7: Outer membrane fraction of pAIDA1 without passenger (positive control). Lane 3: pAIDA1-SefA soluble fraction. Lane 4: pAIDA1-SefA inner membrane fraction. Lane 5: pAIDA1-SefA outer membrane fraction. Lane 8: pAIDA1-H:gm soluble fraction. Lane 9: pAIDA1-H:gm inner membrane fraction. Lane 10: pAIDA1-H:gm outer membrane fraction. Right: Detection through an anti-Myc antibody conjugated with horseradish peroxidase. Lanes 1 and 6: Size marker. Lane 2: pAIDA1-SefA soluble fraction. Lane 3: pAIDA1-SefA inner membrane fraction. Lane 4: pAIDA1-SefA outer membrane fraction. Lane 5 and 10: Outer membrane fraction of pAIDA1 without passenger (positive control). Lane 7: pAIDA1-H:gm soluble fraction. Lane 8: pAIDA1-H:gm inner membrane fraction. Lane 9: pAIDA1-H:gm outer membrane fraction.