Figure 3
From: A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli
Flow cytometry histograms of expressed H:gm on the surface of E. coli . Left: Detection of His6-tag by the THETM His Tag [FITC] antibody. Negative control (red) consisting of cells lacking vector showed a mean fluorescence of 0.6. Cells harboring pDT1-H:gm (green) showed a mean fluorescence of 1.4. Cells harboring pAIDA1-H:gm (blue) show a mean fluorescence of 1.5. Right: Detection of Myc-tag by Anti-c-Myc [SureLight® Allophycocyanin] antibody. Negative control (red) showed a mean fluorescence of 0.6 and cells harboring pAIDA1-H:gm (blue) show a mean fluorescence of 7.6.