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Figure 2 | Microbial Cell Factories

Figure 2

From: A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli

Figure 2

Flow cytometry histograms of expressed SefA on the surface of E. coli . Left: Detection of His6 tag by the THETM His Tag [FITC] antibody. Negative control (red) consisting of cells lacking vector showed a mean fluorescence of 0.6. Cells harboring pDT1-SefA (green) showed a mean fluorescence of 10.5 in the positive population. Cells harboring pAIDA1-SefA (blue) show a mean fluorescence of 15.3. Right: Detection of Myc tag by Anti-c-Myc [SureLight® Allophycocyanin] antibody. Negative control (red) showed a mean fluorescence of 0.6 and cells harboring pAIDA1-SefA (blue) show a mean fluorescence of 15.4.

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