Figure 1
From: A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli
Schematic representations of pAIDA1 and the surface expressed fusion proteins of pAIDA1 and pDT1. Left: The surface expression vector pAIDA1 based on the AIDA-I autotransporter. Expression is under the control of the lacUV5 promoter, and two detection tags (His6 and Myc) with adjacent protease cleavage recognition sites (for 3C and TEV) flank the passenger protein. Top right: Matured fusion protein at the cell surface from vector pAIDA1. Bottom right: Matured fusion protein at the cell surface from vector pDT1.