Table 2 Plasmids used in this study
Plasmid name | Plasmid description | Reference |
|---|---|---|
pSP-GM2 | URA3-based expression plasmid carrying a bidirectional P TEF1 -P PGK1 promoter | [55] |
pICK01 | P TEF1 -SanSyn, P PGK1 -tHMG1 | [12] |
pISP15 | P TEF1 -SanSyn opt , P PGK1 -tHMG1 | this study |
pIGS01 | P TEF1 -ERG20 | this study |
pIGS02 | P TEF1 -ERG20, AD1 | this study |
PIGS03 | P TEF1 -ERG20, P PGK1 -GDH2, AD1 | this study |
pIGS04 | P TEF1 -ERG20, P PGK1 -GDH2, AD1, AD2 | this study |
pIGS05 | P TEF1 -ERG20, P PGK1 -GDH2, AD1, AD2, KlURA3 | this study |
pIGS06 | P TEF1 -ERG20 P PGK1 -GDH2, AD1, 5´KlURA3 | this study |
pIGS07 | P TEF1 -tHMG1 | this study |
pIGS08 | P TEF1 -tHMG1, P PGK1 -upc2-1 | this study |
pIGS09 | P TEF1 -tHMG1, P PGK1 -upc2-1, AD3 | this study |
pIGS10 | P TEF1 -tHMG1, P PGK1 -upc2-1, AD3, 3´KlURA3 | this study |