Combined metabolic engineering of precursor and co-factor supply to increase α-santalene production by Saccharomyces cerevisiae

Table 1 Physiological parameters measured during double-phase chemostat cultures of strains SCIGS28, SCIGS29, SCIGS30, SCIGS31, SCIGS24 and SCIGS25

Strain	D	Y _xs	r_s	r_CO2	r_O2	r_etoh	r_acet	RQ	C_s	C_balance	Tot_sant
	(h^-1)	(g g^-1)	(mmol g biomass^-1 h^-1)					(r_CO2/r_O2)	(mM)	(%)	(mg 24 h^-1 l^-1)
SCIGS28	0.051 ±0.002	0.50 ±0.01	0.57 ±0.01	1.12 ±0.08	1.12 ±0.06	0	0	1.00 ±0.02	0.18 ±0.02	100.3 ±2.1	11.8 ±0.3
SCIGS28	0.10 ±0.01	0.50 ±0.01	1.11 ±0.03	2.67 ±0.15	2.49 ±0.07	0	0	1.07 ±0.05	0.16 ±0.01	101.9 ±1.1	5.2 ±0.2
SCIGS29	0.050 ±0.003	0.29 ±0.01	0.97 ± 0.04	3.17 ±0.04	2.95 ±0.15	0.082 ±0.001	0.024 ±0.001	1.07 ±0.07	1.16 ±0.04	96.9 ±3.2	37.6 ±0.2
SCIGS29	0.10 ±0.01	0.28 ±0.01	1.95 ±0.05	6.40 ±0.18	5.51 ±0.20	0.128 ±0.004	0.039 ±0.008	1.16 ±0.22	0.39 ±0.01	95.0 ±0.9	20.4 ±0.1
SCIGS30	0.051 ±0.001	0.28 ±0.02	1.09 ±0.05	3.60 ±0.09	3.41 ±0.22	0.099 ±0.015	0.024 ±0.003	1.05 ±0.09	1.15 ±0.04	94.7 ±0.4	39.4 ±0.4
SCIGS30	0.10 ±0.02	0.25 ±0.01	2.26 ±0.09	7.70 ±0.19	6.37 ±0.29	0.161 ±0.007	0.032 ±0.005	1.21 ±0.14	0.40 ±0.01	93.5 ±1.2	20.1 ±0.1
SCIGS31	0.051 ±0.001	0.33 ±0.01	0.86 ±0.01	2.31 ±0.08	1.42 ±0.23	0.501 ±0.077	0.047 ±0.007	1.62 ±0.11	30.67 ±0.78	105.4 ±4.9	6.1 ±0.1
SCIGS24	0.051 ±0.001	0.24 ±0.01	1.16 ± 0.03	3.85 ±0.05	3.21 ±0.07	0.185 ±0.003	0.020 ±0.008	1.20 ±0.02	2.53 ±0.09	93.7 ±5.3	26.9 ±0.2
SCIGS25	0.048 ±0.003	0.21 ±0.01	1.26 ±0.02	4.41 ±0.04	3.82 ±0.04	0.195 ±0.005	0.027 ±0.007	1.16 ±0.03	2.91 ±0.14	93.8 ±2.9	24.9 ±0.3

μ_max, specific growth rate (h^-1); D, dilution rate (h^-1); Ysx, biomass yield (g biomass (g substrate)^-1); specific consumption rates of glucose(r_s), and oxygen (r_O2) (mmol (g biomass)^-1 h^-1); specific production rates of carbon dioxide (r_CO2), ethanol (r_etoh),and acetate (r_acet) (mmol (g biomass)^-1 h-¹). RQ, respiratory quotient r_CO2/r_O2; C_s, residual glucose concentration (mM); C_balance, carbon recovery (%); Tot_sant total amount of α-santalene produced (mg 24 h^-1 l^-1). Values represent the mean ± S.D. of three independent cultivations.

ISSN: 1475-2859