Table 3 Functional group assignment of FT-IR spectra (adapted from[17])
Wave number (cm-1) | Functional group assignment |
|---|---|
1739-1725 | stretching CdO of ester functional groups from membrane lipids and fatty acids; stretching CdO of carboxylic acoid |
1647 | stretching CdO in amides (amide I band); bending -NH and -NH2 of amines |
1548 | N-H bending and C-N stretching in amides (amide II band); bending -NH and -NH2 of amines |
1402 | symmetric stretching for deprotonated COO- group |
1453 | bending CH2/CH3 (scissoring) |
1384 | symmetric stretching of COO-; bending CH2/CH3 |
1305 | vibration C-N from amides |
1300-1250 | vibrations of C-O from esters or carboxylic acids |
1262 | vibrations of -COOH and C-O-H; double bond stretching of > PdO of general phosphoryl groups and phosphodiester of nucleic acids |
1225 | stretching of PdO in phosphodiester of nucleic acids |
1225 | stretching CdO in phosphates |
1200-950 | asymmetric and symmetric stretching of PO2- and P(OH)2 in phosphate; vibration of C-OH, C-O-C, and C-C of polysaccharides |
1085 | stretching PdO of phosphodiester, phosphorylated proteins, or polyphosphate products |
976 | symmetric stretching vibration of phosphoryl groups |