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Table 4 Biochemical properties of L-arabinose isomerases from various microorganisms

From: A method for the production of D-tagatose using a recombinant Pichia pastoris strain secreting β-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c

Organism Molecular mass (kDa) Oligomeric state Topt(°C) pHopt Metal ion requirement References
Arthrobacter sp. 22c 335 (55.2)a 6 47-52 5.0-9.0 Not required This study
Lactobacillus sakei 23 K 220 (53.7)a 4 30-40 5.0-7.0 Mn2+, Mg2+ 32
Escherichia coli 362 (56.0)a 6 30 8.0 Fe2+, Mn2+ 18, 37
Bacillus halodurans 224 (56.3)a 4 50 7.5-8.0 Not required 19
Lactobacillus plantarum NC8 342 (53.6)a 6 60 7.5 Mn2+, Co2+ 20
Thermus sp. IM6501 (56.0)a NR 60 8.5 Mn2+ 27
Lactobacillus fermentum CGMCC2921 (53.4)a NR 65 6.5 Mn2+, Co2+ 21
Bacillus stearothermophilus IAM 11001 (55.9)a NR 65 7.5 Mn2+ 24
Alicyclobacillus acidocaldarius 224 (56.0)a 4 65 6.0-6.5 Mn2+, Co2+, Mg2+ 25
Thermoanaerobacter mathranii 220 (52.8)a 4 65 8.0 Mn2+ 29
Geobacillus stearothermophilus 224 (56.1)a 4 70 7.0-7.5 Mn2+, Co2+, Mg2+ 19
Geobacillus thermodenitrificans 230 (56.1)a 4 70 8.5 Mn2+ 22
Acidothermus cellulolytics ATCC 43068 (54.8)a NR 75 7.5 Mn2+, Co2+ 28
Bacillus stearothermophilus US100 225 (56.2)a 4 80 7.5-8.0 Mn2+b, Co2+b 23
Thermotoga neapolitana (56.7)a NR 85 7.0 Mn2+, Co2+ 30
Thermotoga maritima 230 (56.7)a 4 90 7.5 Mn2+, Co2+ 31
Anoxybacillus flavithermus TC-06 (55.9)a NR 95 9.5-10.5 Ni2+ 26
  1. NR not reported; a molecular mass of the monomer, calculated from the amino acid sequence; b metal ions required only for thermostability[37].