A method for the production of D-tagatose using a recombinant Pichia pastoris strain secreting β-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c

Table 4 Biochemical properties of L-arabinose isomerases from various microorganisms

Organism	Molecular mass (kDa)	Oligomeric state	T_opt(°C)	pH_opt	Metal ion requirement	References
Arthrobacter sp. 22c	335 (55.2)^a	6	47-52	5.0-9.0	Not required	This study
Lactobacillus sakei 23 K	220 (53.7)^a	4	30-40	5.0-7.0	Mn²⁺, Mg²⁺	32
Escherichia coli	362 (56.0)^a	6	30	8.0	Fe²⁺, Mn²⁺	18, 37
Bacillus halodurans	224 (56.3)^a	4	50	7.5-8.0	Not required	19
Lactobacillus plantarum NC8	342 (53.6)^a	6	60	7.5	Mn²⁺, Co²⁺	20
Thermus sp. IM6501	(56.0)^a	NR	60	8.5	Mn²⁺	27
Lactobacillus fermentum CGMCC2921	(53.4)^a	NR	65	6.5	Mn²⁺, Co²⁺	21
Bacillus stearothermophilus IAM 11001	(55.9)^a	NR	65	7.5	Mn²⁺	24
Alicyclobacillus acidocaldarius	224 (56.0)^a	4	65	6.0-6.5	Mn²⁺, Co²⁺, Mg²⁺	25
Thermoanaerobacter mathranii	220 (52.8)^a	4	65	8.0	Mn²⁺	29
Geobacillus stearothermophilus	224 (56.1)^a	4	70	7.0-7.5	Mn²⁺, Co²⁺, Mg²⁺	19
Geobacillus thermodenitrificans	230 (56.1)^a	4	70	8.5	Mn²⁺	22
Acidothermus cellulolytics ATCC 43068	(54.8)^a	NR	75	7.5	Mn²⁺, Co²⁺	28
Bacillus stearothermophilus US100	225 (56.2)^a	4	80	7.5-8.0	Mn^2+b, Co^2+b	23
Thermotoga neapolitana	(56.7)^a	NR	85	7.0	Mn²⁺, Co²⁺	30
Thermotoga maritima	230 (56.7)^a	4	90	7.5	Mn²⁺, Co²⁺	31
Anoxybacillus flavithermus TC-06	(55.9)^a	NR	95	9.5-10.5	Ni²⁺	26

NR not reported; ^a molecular mass of the monomer, calculated from the amino acid sequence; ^b metal ions required only for thermostability[37].

ISSN: 1475-2859