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Figure 2 | Microbial Cell Factories

Figure 2

From: A method for the production of D-tagatose using a recombinant Pichia pastoris strain secreting β-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c

Figure 2

SDS-PAGE analysis of the fractions obtained by expression and purification of Arthrobacter sp. 22c L-arabinose isomerase. Lane 1 – Unstained Protein Molecular Weight Marker (Fermentas): 116, 66.2, 45, 35, 25, 18.4 and 14.4 kDa, lane 2 – cell extract of E. coli BL21(DE3)pLysS + pET30araA22c after araA gene expression, lane 3 – purified L-arabinose isomerase after ion exchange chromatography on Fractogel EMD DEAE column, lane 4 - purified L-arabinose isomerase after ion exchange chromatography on Fractogel EMD TMAE column.

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