A novel, lactase-based selection and strain improvement strategy for recombinant protein expression in Kluyveromyces lactis

Table 1 Kluyveromyces lactis strains

Name	Parent	Relevant genotype	Heterologous gene	Reference
RUL1888	CBS2356	Klura3-59	none	H.Y. Steensma (Univ. Leiden NL)
RUL1888-D4	RUL1888	lac4::ScURA3	ScURA3	this work
RULOx	RUL1888-D4	P _LAC4 -scFv _ox	scFv _ox ^a	this work
RULOx/D802	RULOx	P _LAC4 -scFv _ox Klgal80Δ2::ScURA3	ScURA3	this work
VAK367	CBS 2359/152	metA ura3-5	none	this work
VAK367-D4	VAK367	lac4::ScURA3	ScURA3	this work
VAK726	VAK367-D4	P _LAC4 -E2-1 _BVDV	E2-1 _BVDV ^b	this work
VAK746	VAK367-D4	P _LAC4 -E2-1 _BVDV Klgal80Δ2::ScURA3	E2-1 _BVDV ^b	this work
VAK834	726VAK	P_LAC4-E2-1_BVDV (pLI-1)	E2-1 _BVDV ^b	this work
VAK836	VAK726	P_LAC4-E2-1_BVDV (pLI-1)₄	E2-1 _BVDV ^b	this work
VAK906	VAK726	P_LAC4-E2opt (pLI-1)_n	E2-opt _BVDV ^c	this work

^a gene encoding oxazolon specific single-chain antibody fragment.
^bE2-1 segment of the bovine viral diarrhea virus (strain CP7) containing the E2 ORF and 5’ and 3’ flanking sequences.
^c yeast codon-optimized synthetic gene corresponding to VSV-E2(MTdel), which lacks the membrane anchor and is derived from BVDV strain NADL[22].

ISSN: 1475-2859