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Figure 6 | Microbial Cell Factories

Figure 6

From: A novel, lactase-based selection and strain improvement strategy for recombinant protein expression in Kluyveromyces lactis

Figure 6

Influence of elevated KlGAL4 gene dosage on E2 and LAC4 mRNA levels. The E2-1-expressing strains VAK834 and VAK836 (Table1) contain one and four tandem copies, respectively, of plasmid pLI-1 carrying KlGAL4 in addition to the endogenous KlGAL4 gene copy. mRNA was prepared from cells grown in 0.5% lactose and quantified by RT-qPCR using KlACT1 as a reference.

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