Figure 4From: A novel, lactase-based selection and strain improvement strategy for recombinant protein expression in Kluyveromyces lactisDetection of E2 protein expression in yeast cells by immunocytology. K. lactis strains VAK367 (A) and VAK726 (B and C), which contains the BVDV-E2 ORF in single copy under control of the LAC4 promoter, were grown under inducing conditions (2% lactose) and analyzed by confocal microscopy after staining with DAPI (panels b), E2-specific antibody WB-214 (PA2020; c.c.pro, Germany) and a polyclonal secondary antibody (Dylight 488; Dianova) (panels c) and Dil18 (Invitrogen) for staining of the plasma membrane (panels d). E2 and nuclear (DAPI) staining is merged in panels a. C) Overlay of DNA, membrane-, and E2- staining reveals accumulation of E2 in perinuclear regions suggesting localization in the ER.Back to article page