Skip to main content
Figure 4 | Microbial Cell Factories

Figure 4

From: A novel, lactase-based selection and strain improvement strategy for recombinant protein expression in Kluyveromyces lactis

Figure 4

Detection of E2 protein expression in yeast cells by immunocytology. K. lactis strains VAK367 (A) and VAK726 (B and C), which contains the BVDV-E2 ORF in single copy under control of the LAC4 promoter, were grown under inducing conditions (2% lactose) and analyzed by confocal microscopy after staining with DAPI (panels b), E2-specific antibody WB-214 (PA2020; c.c.pro, Germany) and a polyclonal secondary antibody (Dylight 488; Dianova) (panels c) and Dil18 (Invitrogen) for staining of the plasma membrane (panels d). E2 and nuclear (DAPI) staining is merged in panels a. C) Overlay of DNA, membrane-, and E2- staining reveals accumulation of E2 in perinuclear regions suggesting localization in the ER.

Back to article page