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Figure 3 | Microbial Cell Factories

Figure 3

From: A novel, lactase-based selection and strain improvement strategy for recombinant protein expression in Kluyveromyces lactis

Figure 3

Leakage of K. lactis proteins into the culture medium. A) Strain RUL1888 was grown for 6 (lane 1), 24 (lane 2), 30 (lane 3) and 48 hrs (lane 4) in YP medium with an initial concentration of 0.04% galactose. B) Strain RUL1888, JA6 and a diploid strain resulting from a cross between JA6/D4 and RUL1888 were grown for 24 (lane 1), 48 (lane 2), 72 (lane 3) and 96 hrs (lane 4) in 1% galactose. Culture supernatants were applied to SDS-PAGE and gels were stained after electrophoresis by Coomassie brilliant blue.

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