Figure 3
From: A genetic replacement system for selection-based engineering of essential proteins
Characterization of the pCOMP- groE /pP ara I- Sce I and the pCOMP-sec BgpsA /pP ara I- Sce I system. Elimination of (A) pCOMP-groE from groE::kan and (B) pCOMP-secBgpsA from secBgpsA::kan in the presence of arabinose and the helper plasmid pParaI-Sce I. Elimination is induced by arabinose-induced expression of I-Sce I nuclease from helper plasmid pParaI-Sce I. Red arrow: Time point for harvest and competent cell preparation.