Figure 7From: Low-mutation-rate, reduced-genome Escherichia coli: an improved host for faithful maintenance of engineered genetic constructsAccumulation of plasmids with mutated sinI in various hosts. SinI methyltransferase was expressed from pSin32. Plasmids were isolated at various intervals and screened (by transformation in McrBC+ and McrBC- hosts) for mutations resulting in loss of function of the enzyme. Error bars represent 95% confidence intervals for the average of 3 independent measurements of mutant plasmid ratios. ANOVA revealed a significance of p < 0.005. Pairwise t-tests of each MDS42pdu-T7 sample were done with the corresponding MDS42-T7 and BL21(DE3)mcrBC sample, respectively. Starting from 10 hours, all MDS42pdu-T7 samples differed significantly from the MDS42-T7 (p < 0.01) or BL21(DE3)mcrBC (p < 0.005) samples.Back to article page