Skip to main content
Figure 4 | Microbial Cell Factories

Figure 4

From: Low-mutation-rate, reduced-genome Escherichia coli: an improved host for faithful maintenance of engineered genetic constructs

Figure 4

Mutation rates of various strains under unstressed and stressful conditions. Stress conditions include overproduction of GFP, overproduction of a toxic peptide from pSG-ORF238, and treatment with mitomycin-C. All measurements were made using the cycA fluctuation assay, error bars represent 95% confidence intervals for the average of 3 independent measurements. BL21(DE3) and MDS42recA failed to grow in the presence of 0.1 μg/ml mitomycin-C. ANOVA revealed a significance of p < 0.0001. Pairwise t-tests were conducted for each strain under a given condition compared to the corresponding MDS42pdu strain, * indicates a significance of p < 0.05, ** indicates a significance of p < 0.01, *** indicates a significance of p < 0.001.

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us