Table 2 Experimental design
From: A novel system for stable, high-level expression from the T7 promoter
Experiment | Expression vector | Markers | Type of experiment | Antibiotic in selective medium | Target protein | |
|---|---|---|---|---|---|---|
Type I | Type II | |||||
B | pGKE2 | CmR | KmR | ♦ experiment | Km | kE2 |
â—Š control | Cm | |||||
C | p7KE2 | AmpR | KmR | ♦ experiment | Km | kE2 |
â—Š control | Amp | |||||
D | pGKPA | CmR | KmR | ♦ experiment | Km | PA-4D |
pGPA | CmR | − | ◊ control | Cm | ||