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Figure 7 | Microbial Cell Factories

Figure 7

From: Development of new plasmid DNA vaccine vectors with R1-based replicons

Figure 7

Plasmid DNA quality assessment. Agarose gel electrophoresis of pDMB02-GFP and pDMB-ATG produced in DH5α with and without a mid-exponential phase temperature shift from 30°C to 42°C. Lane 1: DNA ladder with DNA size in kilobases (kb) indicated to the left of the image, Lane 2: pDMB02-GFP t = 2 hr 30°C, Lane 3: pDMB02-GFP t = 2 hr 42°C, Lane 4: pDMB02-GFP t = 4 hr 30°C, Lane 5: pDMB02-GFP t = 4 hr 42°C, Lane 6: pDMB02-GFP t = 8 hr 30°C, Lane 7: pDMB02-GFP t = 8 hr 42°C, Lane 8: pDMB-ATG t = 2 hr 30°C, Lane 9: pDMB-ATG t = 2 hr 42°C, Lane 10: pDMB-ATG t = 4 hr 30°C, Lane 11: pDMB-ATG t = 4 hr 42°C, Lane 12: pDMB-ATG t = 8 hr 30°C, Lane 13: pDMB-ATG t = 8 hr 42°C, Lane 14: DNA ladder. Times refer to hours after the temperature shift.

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