Figure 4
From: Development of new plasmid DNA vaccine vectors with R1-based replicons
Specific yield of pDMB02-GFP as a function of inoculum growth phase. Specific yield after 24 hours of culture at 30°C in shake flasks is shown (gray bars). The plasmid copy numbers of the seed cultures at each growth phase are also shown (▪). Specific yield error bars represent one standard deviation calculated from duplicate flasks, and copy number error bars represent the 95% confidence interval calculated from three replicate wells of the same sample.