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Figure 1 | Microbial Cell Factories

Figure 1

From: Development of new plasmid DNA vaccine vectors with R1-based replicons

Figure 1

Feature map of pDMB02-GFP. DNA vaccine vector features: Kanamycin resistance marker (KanR), human cytomegalovirus immediate-early promoter/enhancer (PCMV), superfolding GFP gene (sGFP), bovine growth hormone polyadenylation signal (BGH PolyA). R1 replicon features: Replication initiation protein gene (repA), antisense RNA gene (copA), repressor of PrepA gene (copB), origin of replication (R1 ori). The point mutations that confer the runaway replication phenotype are also indicated (RR1, RR2).

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