Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes

Table 1 Used Strains and their expression plasmid copy number determined by RT-PCR

Strains	Description	Copy number	Reference
H222-S4	MATA ura3-302 ^a		[76]
	(Ura^-, Alk⁺, Tgl⁺, Lip⁺, Eth⁺, Glu⁺,Suc⁺)		[76]
YL23	H222-S4 transformed with SacII – linearized p65D-linker plasmid, negative control		This work
YL21	H222-S4 transformed with SacII - linearized p64D-hCPRwt-2D6syn plasmid	46 ± 4	This work
YL10	H222-S4 transformed with SacII - linearized p64D-hCPRsyn-2D6syn plasmid	30 ± 3	This work
YL22	H222-S4 transformed with SacII - linearized p64D-hCPRwt-3A4syn plasmid	23 ± 2	This work
YL11	H222-S4 transformed with SacII - linearized p64D-YlCPR-2D6syn plasmid	26 ± 2	This work
YL18	H222-S4 transformed with SacII - linearized p64D-YlCPR-3A4syn plasmid	10 ± 1	This work
YL15	H222-S4 transformed with SacII - linearized p65D-hCPRwt-2D6syn plasmid	0.9 ± 0.1	This work
YL20	H222-S4 transformed with SacII - linearized p65D-hCPRwt-3A4syn plasmid	1.1 ± 0.1	This work
YL12	H222-S4 transformed with SacII - linearized p65D-YlCPR-2D6syn plasmid	1.1 ± 0.1	This work
YL19	H222-S4 transformed with SacII - linearized p65D-YlCPR-3A4syn plasmid	1.1 ± 0.1	This work

^aura3-302: URA3 disrupted by a construct pXPR2 SUC2 (invertase gene from S. cerevisiae). This allele confers the ability to grow on sucrose or mollasses[76, 77].

ISSN: 1475-2859