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Table 1 Used Strains and their expression plasmid copy number determined by RT-PCR

From: Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes

Strains Description Copy number Reference
H222-S4 MATA ura3-302 a   [76]
  (Ura-, Alk+, Tgl+, Lip+, Eth+, Glu+,Suc+)  
YL23 H222-S4 transformed with SacII – linearized p65D-linker plasmid, negative control   This work
YL21 H222-S4 transformed with SacII - linearized p64D-hCPRwt-2D6syn plasmid 46 ± 4 This work
YL10 H222-S4 transformed with SacII - linearized p64D-hCPRsyn-2D6syn plasmid 30 ± 3 This work
YL22 H222-S4 transformed with SacII - linearized p64D-hCPRwt-3A4syn plasmid 23 ± 2 This work
YL11 H222-S4 transformed with SacII - linearized p64D-YlCPR-2D6syn plasmid 26 ± 2 This work
YL18 H222-S4 transformed with SacII - linearized p64D-YlCPR-3A4syn plasmid 10 ± 1 This work
YL15 H222-S4 transformed with SacII - linearized p65D-hCPRwt-2D6syn plasmid 0.9 ± 0.1 This work
YL20 H222-S4 transformed with SacII - linearized p65D-hCPRwt-3A4syn plasmid 1.1 ± 0.1 This work
YL12 H222-S4 transformed with SacII - linearized p65D-YlCPR-2D6syn plasmid 1.1 ± 0.1 This work
YL19 H222-S4 transformed with SacII - linearized p65D-YlCPR-3A4syn plasmid 1.1 ± 0.1 This work
  1. aura3-302: URA3 disrupted by a construct pXPR2 SUC2 (invertase gene from S. cerevisiae). This allele confers the ability to grow on sucrose or mollasses[76, 77].