Table 1 Kinetic parameters of Tth PAC for the hydrolysis of different substrates
Substrate | R (acyl chain) | Km(mM) | kcat | kcat/Km |
|---|---|---|---|---|
Penicillin K | Octanoyl | 0.32 ± 0.05 | 5.16 ± 0.13 | 16.12 |
Penicillin dihydro F | Hexanoyl | 0.98 ± 0.22 | 1.46 ± 0.06 | 1.49 |
Penicillin F | hexenoyl | 0.94 ± 0.18 | 0.90 ± 0.03 | 0.96 |
Penicillin V | Phenoxyacetyl | 0.74 ± 0.11 | 0.57 ± 0.01 | 0.77 |
Penicillin G | Phenylacetyl | Approx. 2.90 | Approx. 4.10-4 | Approx. 1.10-4 |
Glutaryl-7-ACA | Glutaryl | nd | nd | nd |
Cephalosporin C | 7-aminoadipyl | nd | nd | nd |
C4-HSL | Butiryl | nd | nd | nd |
C8-HSL | Octanoyl | nd | nd | nd |