Skip to main content

Advertisement

Table 1 Kinetic parameters of Tth PAC for the hydrolysis of different substrates

From: Functional expression of a penicillin acylase from the extreme thermophile Thermus thermophilus HB27 in Escherichia coli

Substrate R (acyl chain) Km(mM) kcat kcat/Km
Penicillin K Octanoyl 0.32 ± 0.05 5.16 ± 0.13 16.12
Penicillin dihydro F Hexanoyl 0.98 ± 0.22 1.46 ± 0.06 1.49
Penicillin F hexenoyl 0.94 ± 0.18 0.90 ± 0.03 0.96
Penicillin V Phenoxyacetyl 0.74 ± 0.11 0.57 ± 0.01 0.77
Penicillin G Phenylacetyl Approx. 2.90 Approx. 4.10-4 Approx. 1.10-4
Glutaryl-7-ACA Glutaryl nd nd nd
Cephalosporin C 7-aminoadipyl nd nd nd
C4-HSL Butiryl nd nd nd
C8-HSL Octanoyl nd nd nd
  1. nd: no detectable activity.